干细胞生长分化因子

产品名称：干细胞生长分化因子

WNT-5a,human W5A-H-005,5ug 产品简介
Wnt信号转导通路因其启动蛋白为Wnt蛋白而得名，在胚胎发育、细胞增殖及分化等方面发挥重要 作用，其异常活化与人类多种肿瘤的发生密切相关。研究显示，Wnt信号至少存在经典和非经典两条不同的信号通路，而Wnt-5a属于非经典Wnt信号通 路，既往研究Ror2具有Wnt受体结构域样结构。有研究认为Wnt-5a经由Ror2介导的 信号通路发挥作用。
Human WNT-5a蛋白含有357个氨基酸残基。

人源WNT-5a 蛋白因子，5ug 产品特性
? 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定，纯度高，无动物成分污染
?人源WNT-5a 蛋白因子，5ug 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定
? 人源WNT-5a 蛋白因子，5ug 产品经过SDS-PAGE 和HPLC双层鉴定纯度大于85%

人源WNT-5a 蛋白因子，5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.

  产品英文描述
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product WNT-5a,human W5A-H-005,5ug is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
?WNT-5a,human W5A-H-005,5ug Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
?WNT-5a,human W5A-H-005,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP-  5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparat

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