用户论文：全自动固相萃取仪在地下水中橡胶添加剂及其氧化产物的应用

本研究对中国中部地上河地区地下水中橡胶添加剂及相关氧化产物（RAROPs）的存在情况进行了调查。检测出了7种RAROPs，其在浅层地下水中的含量从黄河附近区域（平均值：8.49 ng L-1）到黄河远岸区域（平均值：5.01 ng L-1）呈轻微下降趋势。相比之下，深层地下水中RAROPs的含量急剧下降，仅为0.26 ng L-1。发现主要污染物为N-（1,3-二甲基丁基）-N'-苯基-对苯二胺（6PPD）。

车库和停车场附近常被视为污染热点区域。相关性分析进一步表明，水产养殖可能是浅层地下水污染物输入的潜在途径。由于城乡发展不平衡，人类通过地下水摄入的RAROPs量相差近30倍。儿童最容易受到RAROPs的侵害。因此，人类活动（交通运输、废旧轮胎储存、水资源分配与利用模式、黄河水引入养殖池塘）可能会通过地表土壤淋滤污染物，加剧地下水中RAROPs的污染。这些结果对于发展中国家制定适宜的地下水资源利用与保护策略具有重要意义。 

Each 300 mL of groundwater sample was filtered through precleaned 0.7 um GF (glass microfiber filter)/F membranes. Then, filtrateswere extracted by high flux automatic solid-phase extractor (FotectolPlus, RayKol Group Corp., Ltd., Xiamen, china) with Oasis hydrophiliclipophilic balanced (HLB) solid-phase extraction (SPE) cartridges (500mg6 mL1), following the proceduredescribed below.

Each cartridge was sequentially pre-conditioned with 10 mL ofmethanol and 10 mL of deionized water. After that, the filtrates wereloaded onto the cartridge at a flow rate of approximately 1.5 mL min-1The cartridge was then rinsed with 10 mL of mixed solvent (equalamount of methanol and deionized water by volume) and dried undernitrogen gas for 30 min. After drying, the analytes were eluted from theHLB cartridge using 15 mL methanol and 10 mL acetonitrile. The elutionwas finally evaporated to 0.5 mL, before being diluted to a final amountof 1 mL using deionized water for analysis using ultra-high-performanceliquid chromatography and mass spectrometry (UHPLC-MS/MS).

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