ECHO显微镜助力活体心脏中胚胎干细胞来源的研究

在2017年12月，由Xulei Qin，Huaxiao Yang，Haodi Wu，Xin Zhao等作者在Advanced Functional Materials中发表一篇了《Photoacoustic Imaging of Embryonic Stem Cell-Derived Cardiomyocytes in Living Hearts with Ultrasensitive Semiconducting Polymer Nanoparticles》的文章。

文章使用Revolve正倒置一体显微镜获得了如下结果：

该图中B是通过Revolve正倒置一体显微镜成像得到的结果 ↑

该图中E、F是通过Revolve正倒置一体显微镜成像得到的结果 ↑

该图是通过Revolve正倒置一体显微镜成像得到的结果 ↑

中文摘要：

人类胚胎干细胞起源的心肌细胞有望成为修复心脏损伤强有力的工具。为了获得好的结果，我们需要追踪心脏移植细胞，因此*的分子成像技术是至关重要的。本研究中发现，将一类含有半导体聚合物（SPs）的光声纳米粒子（PANPS）作为造影剂，可用于活体小鼠心脏移植hESC-CMs的光声成像（PAI）。这得益于PANPs的两个优势。首先，强光声信号（PA）和SPs特定光谱曲线使得PAI从背景组织中检测和区分少量PANP标记的细胞（2000）具有很高的灵敏度。其次，PANP对hESC-CM的标记效率高，并且也不影响细胞的结构、功能和基因表达。通过超声成像，我们可以利用PANP依赖的高空间分辨率（≈100um）的PAI来评估hESC-CM在活体小鼠心脏中的移植状态。综上所述，本研究探索并验证了SPs新的应用方向，可作为PA造影剂实现对体内标记细胞高灵敏度、高准确度的示踪，凸显了PAI与PANPs结合在心脏再生治疗上的优势。

英文摘要：

Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) have become promising tools to repair injured hearts. To achieve optimal outcomes,advanced molecular imaging methods are essential to accurately track these transplanted cells in the heart. In this study, it is demonstrated for the first time that a class of photoacoustic nanoparticles (PANPs) incorporating semiconducting polymers (SPs) as contrast agents can be used in the photoacoustic imaging (PAI) of transplanted hESC-CMs in living mouse hearts. This is achieved by virtue of two benefits of PANPs. First, strong photoacoustic (PA) signals and specific spectral features of SPs allow PAI to sensitively detect and distinguish a small number of PANP-labeled cells(2000) from background tissues. Second, the PANPs show a high efficiency for hESC-CM labeling without adverse effects on cell structure, function, and gene expression. Assisted by ultrasound imaging, the delivery and engraftment of hESC-CMs in living mouse hearts can be assessed by PANP-based PAI with high spatial resolution (≈100 μm). In summary, this study explores and validates a novel application of SPs as a PA contrast agent to track labeled cells with high sensitivity and accuracy in vivo, highlighting the advantages of integrating PAI and PANPs to advance cardiac regenerative therapies.

ECHO Revolve正倒置一体显微镜展现了其非凡的灵活性，可以轻松地实现正置和倒置显微镜转换，创新性地把正倒置显微镜合二为一，开启了显微镜Hybrid时代。

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