文献分享 | ECHO 正倒置显微镜助力小檗碱诱导癌细胞周期阻滞的研究

在2017年11月11日，由Fanni Li，Wenxi Dong，Peng Lin和Jianli Jiang在Biology of Reproduction中发表一篇了《Regulation of Akt/FoxO3a/Skp2 Axis Is Critically Involved in Berberine-Induced Cell Cycle Arrest in Hepatocellular Carcinoma Cells》的文章。

文章使用ECHO Revolve正倒置一体显微镜获得了如下结果:

中文摘要：

ECHO Revolve正倒置一体显微镜观察到的有序的细胞周期是肿瘤发生中关键的生物学过程，是抗癌药物的重要靶点。小檗碱（BBR）是一种重要的天然异喹啉类生物碱，具有抗癌潜能，可诱导肝癌（HCC）细胞周期阻滞。然而，其基本机制尚待阐明。在我们目前研究中，观察到小檗碱处理的HUH-7和HepG2细胞G0/G1细胞周期阻滞。简单的说，BBR可使AKT通路失活，从而抑制S期激酶相关蛋白SKP2的表达，并增强FoxO3a在细胞核中的表达和转运。易位的FXO3a一方面能直接促进细胞周期蛋白依赖性激酶抑制剂（CDKIS）p21^CIP1和p27^kip1的转录，另一方面抑制Skp2的表达，导致p21^CIP1和p27^kip1上调，肝癌HCC G0/G1细胞周期阻滞。综上所述，BBR通过调节Akt/FoxO3A/Skp2促进CDKIs p21^CIP1和p27^kip1的表达，诱导肝癌HCC G0/G1细胞周期阻滞。本研究揭示了BBR抗癌作用的新机制。

英文摘要：

The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to

induce cell cycle arrest in hepatocellular carcinoma cells (HCC). However, the underlying mechanism remains to be elucidated. In our present study, G0/G1 phase cell cycle arrest was observed in berberine-treated Huh-7 and HepG2 cells. Mechanically, we observed that BBR could deactivate the Akt pathway, which consequently suppressed the S-phase kinase-associated protein 2 (Skp2) expression and enhanced the expression and translocation of Forkhead box O3a (FoxO3a) into nucleus. The translocated FoxO3a on one hand could directly promote the transcription of cyclin-dependent kinase inhibitors (CDKIs) p21^Cip1 and p27^Kip1, on the other hand, it could repress Skp2 expression, both of which lead to up-regulation of p21^Cip1 and p27^Kip1, causing G0/G1 phase cell cycle arrest in HCC. In conclusion, BBR promotes the expression of CDKIs p21^Cip1 and p27^Kip1 via regulating the Akt/FoxO3a/Skp2 axis and further induces HCC G0/G1 phase cell cycle arrest. This research uncovered a new mechanism of an anti-cancer effect of BBR. 

ECHO Revolve正倒置一体显微镜展现了其非凡的灵活性，可以轻松地实现正置和倒置显微镜转换，创新性地把正倒置显微镜合二为一，开启了显微镜Hybrid时代。

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ECHO Revolve正倒置一体显微镜兼具正置和倒置显微镜的优势，方便小巧，一机多能，可以非常便利地通过旋转实现正倒置配置切换；以iPad桌面替代目镜设计, 获得的视觉体验；同时基于iOS的Echo app，使软件操作更人性化。